Journal: Developmental Cell

Article Title: Functional Heterogeneity within the Developing Zebrafish Epicardium

doi: 10.1016/j.devcel.2020.01.023

Figure Lengend Snippet:

Article Snippet: RNase Inhibitor , Clontech , Cat# 2313A.

Techniques: Recombinant, Reverse Transcription, Picogreen Assay, Isolation, Imaging, Cloning, Single Cell, Software

Journal: PLoS ONE

Article Title: Equine Chorionic Gonadotropin Modulates the Expression of Genes Related to the Structure and Function of the Bovine Corpus Luteum

doi: 10.1371/journal.pone.0164089

Figure Lengend Snippet: Antibodies used for Western blotting and Immunohistochemistry.

Article Snippet: ANG , Rabbit polyclonal IgG , 101–200 human ANG protein , 1:1000 , Biorbyt (orb101736).

Techniques: Western Blot, Immunohistochemistry, Purification

Journal: Oncogene

Article Title: RNH1 regulation of reactive oxygen species contributes to histone deacetylase inhibitor resistance in gastric cancer cells.

doi: 10.1038/onc.2013.104

Figure Lengend Snippet: Figure 2. RNH1 is highly expressed in HDACi resistant gastric lines. (a) Left: sensitive and resistant gastric lines were profiled on two different microarray platforms (Affymetrix, Illumina). Middle: genes commonly identified on both platforms as being differentially regulated between sensitive and resistant lines were identified (Po0.05). Right: the bar chart of the top seven common genes (Po0.05, false discovery rate (FDR)o0.3). (b) Western blots showing the protein levels of six candidate genes in sensitive and resistant lines. RNH1 and STAT1 are highlighted in bold type. BLCAP was excluded from analysis because of poor quality of the antibodies.

Article Snippet: RNH1 shRNA transfections and overexpression YCC3 and YCC7 cells were transfected with either RNH1 targeting shRNAs (OriGene Technologies, Rockville, MD, USA) or negative control scrambled shRNAs.

Techniques: Microarray, Western Blot

Journal: Oncogene

Article Title: RNH1 regulation of reactive oxygen species contributes to histone deacetylase inhibitor resistance in gastric cancer cells.

doi: 10.1038/onc.2013.104

Figure Lengend Snippet: Figure 4. RNH1 overexpression renders HDACi-sensitive cells resistant to HDACi treatment. (a) Proliferation assays comparing GI50 values of parental AZ521 or YCC11 cells with AZ521 or YCC11 cells overexpressing RNH1 or empty vector transfected controls. RNH1-overexpressing AZ521 or YCC11 cells exhibited significantly increased GI50 values for TSA, SAHA and MS275 compared with control cells. *Po0.001 between RNH1-overexpressing AZ521 or YCC11 and empty vector control cells. (b) Apoptosis assays comparing RNH1-overexpressing AZ521 or YCC11 cells with empty vector controls before and after HDACi treatment. Western blots were performed to monitor PARP cleavage before and after TSA (200 nM, 24 h), SAHA (5 mM, 24 h) or MS275 treatment (5 mM, 24 h). (c) Colony formation assays comparing RNH1-overexpressing AZ521 or YCC11 cells with empty vector control cells after HDACi treatment. HDACi-treated RNH1-overexpressing AZ521 or YCC11 cells exhibited significant more colony formation after TSA (100 nM, 3 weeks), SAHA (1 mM, 3 weeks) and MS275 treatment (1 mM, 3 weeks) compared with HDACi-treated control AZ521 or YCC11 cells. *Po 0.001 between control and HDACi-treated cells. (d) Colony images of AZ521 cells with or without RNH1 overexpression before or after TSA, SAHA or MS275 treatment. Similar images of YCC11 cells can be found in Supplementary Figure 6.

Article Snippet: RNH1 shRNA transfections and overexpression YCC3 and YCC7 cells were transfected with either RNH1 targeting shRNAs (OriGene Technologies, Rockville, MD, USA) or negative control scrambled shRNAs.

Techniques: Over Expression, Plasmid Preparation, Transfection, Control, Western Blot

Journal: Oncogene

Article Title: RNH1 regulation of reactive oxygen species contributes to histone deacetylase inhibitor resistance in gastric cancer cells.

doi: 10.1038/onc.2013.104

Figure Lengend Snippet: Figure 6. RNH1 levels correlate with TSA sensitivity in normal gastric epithelial cells. (a) Western blots showing RNH1 protein levels in normal gastric epithelial cell lines, HFE145 and GES1. AZ521 (sensitive) and YCC3 (resistant) were included as a reference. (b) Proliferation assays showing TSA GI50 values of HFE145 and GES1 cells. AZ521 and YCC3 were included as reference.

Article Snippet: RNH1 shRNA transfections and overexpression YCC3 and YCC7 cells were transfected with either RNH1 targeting shRNAs (OriGene Technologies, Rockville, MD, USA) or negative control scrambled shRNAs.

Techniques: Western Blot